|
KMID : 0984920140160020100
|
|
Journal of Skin Barrier Research
2014 Volume.16 No. 2 p.100 ~ p.100
|
|
|
Its Impact in Epidermal Differentiation and Possible Role in Treatment of Psoriasis
|
|
Kim Ji-Seok
Nam Chan-Hee
Yoo Ji-Yeon
Kim Hyun-Jung
Jeong Se-Kyoo
Ahn Sung-Ku
Hong Seung-Phil
|
|
|
Abstract
|
|
|
Purpose: There are researches indicating that in human skin, the cannabinoid receptors exist, which take a part of endocannabinoid system. Nonetheless, the possible association of cannabinoid receptors with the pathogenesis of psoriasis has not yet been fully elucidated. Through this research, we tried to document the association between cannabinoid system, epidermal differentiation and psoriasis.
Methods: Using human keratinocyte (KC), the expression of cannabinoid receptor-1 (CB1R) and -2 (CB2R) was analyzed according to the degree of differentiation. Also we examined for changes in differentiation marker of KC after application of CBR agonist. In addition, murine model applied with imiquimod to induce psoriasis symptoms was used to evaluate the effect of topical CBR agonist on inflammation and skin barrier function.
Results: Compared to normal human skin, CBR expression was reduced in epidermis of psoriasis skin. Western blotting revealed the expression of both CB1R and CB2R in undifferentiated KC. The expression level of CB1R increased as the differentiation progressed in KC as with involucrin, K1 and K10. On the other hand, there was no change of CB2R in the process of differentiation. Furthermore, CB1R agonist partly increased expression of proteins associated with epidermal differentiation. Through this we could speculate that the CB system may be associated with proteins related to epidermal differentiation. Moreover, inflammation and barrier function in murine models with psoriasis symptoms were improved after 3 days-application of topical CB1R agonist.
Conclusion: Cannabinoid receptors in human skin might be related to the differentiation of KC, and since its activation could improve skin barrier condition in murine psoriasis model, it can be expected to be a new therapeutic target for treatment of psoriasis.
|
|
|
KEYWORD
|
|
|
Cannabinoid receptor, Epidermal differentiation, Psoriasis, Skin barrier, Epidermal permeability barrier
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|